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MTT Assay for Nanoparticle Cytotoxicity Assessment

Biological Protocol Public PROTO-2026-01-005
By Medic Tech
Updated 1 hour ago • 66 views

Basic Information

Cell viability assay protocol for evaluating the biocompatibility of nanomaterials using the MTT colorimetric method.

Abstract

This protocol describes the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay for assessing nanoparticle cytotoxicity in cultured cells. The method quantifies cell viability based on mitochondrial metabolic activity and provides IC₅₀ values for nanoparticle formulations. The protocol includes cell seeding, nanoparticle treatment, MTT incubation, and colorimetric measurement.

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How to Cite This Protocol

APA

Medic Tech. (2026). MTT Assay for Nanoparticle Cytotoxicity Assessment. Protocol ID: PROTO-2026-01-005. Retrieved from https://protocol.medic.net.in/view/mtt-assay-for-nanoparticle-cytotoxicity-assessment

MLA

Medic Tech. "MTT Assay for Nanoparticle Cytotoxicity Assessment." Protocol ID PROTO-2026-01-005, 2026. Web. 10 Jan 2026.

Chicago

Medic Tech. "MTT Assay for Nanoparticle Cytotoxicity Assessment." Protocol ID: PROTO-2026-01-005. Accessed January 10, 2026. https://protocol.medic.net.in/view/mtt-assay-for-nanoparticle-cytotoxicity-assessment.

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